The world often feels rigged. And this episode is a wake-up call to recognize the barriers that exist for those who don’t fit the traditional mold. In this episode, which is a kind of tribute to my dear departed Dad, I recount some powerful lessons from the man who was a brilliant psychiatrist and my biggest champion. He taught me that if something feels off about the environment you’re in, it probably is—and it’s absolutely hella-not your fault. We dare to break into the uncomfortable truth that many workplaces are designed for a very specific demographic, leaving neurodivergent individuals, particularly those on the autism spectrum, feeling excluded. I share three stories in which my Dad imparted to me more than my fair share of his wisdom, and I'm hoping you to can feel empowered. You'll learn that we can advocate for ourselves and others to create a more inclusive work culture. Newsletter Paste this into your browser if the newsletter link is broken - https://www.lbeehealth.com/ Join our Patreon - https://differentnotbrokenpodcast.com/patreon Mentioned in this episode: Sign Up For Our Newsletter Stay updated on all the things! Get added to our newsletter mailing list. Newsletter…
Player FM - Internet Radio Done Right
Checked 12d ago
اضافه شده در two سال پیش
محتوای ارائه شده توسط Dr. Synaptologica. تمام محتوای پادکست شامل قسمت‌ها، گرافیک‌ها و توضیحات پادکست مستقیماً توسط Dr. Synaptologica یا شریک پلتفرم پادکست آن‌ها آپلود و ارائه می‌شوند. اگر فکر می‌کنید شخصی بدون اجازه شما از اثر دارای حق نسخه‌برداری شما استفاده می‌کند، می‌توانید روندی که در اینجا شرح داده شده است را دنبال کنید.https://fa.player.fm/legal
Player FM - برنامه پادکست
با برنامه Player FM !
با برنامه Player FM !
))
Daily Deals
18. In-depth analysis of negative controls: using Nobel Laureates’ as example (mini-series: reading-11)
Manage episode 409865497 series 3493940
محتوای ارائه شده توسط Dr. Synaptologica. تمام محتوای پادکست شامل قسمت‌ها، گرافیک‌ها و توضیحات پادکست مستقیماً توسط Dr. Synaptologica یا شریک پلتفرم پادکست آن‌ها آپلود و ارائه می‌شوند. اگر فکر می‌کنید شخصی بدون اجازه شما از اثر دارای حق نسخه‌برداری شما استفاده می‌کند، می‌توانید روندی که در اینجا شرح داده شده است را دنبال کنید.https://fa.player.fm/legal
Today's focus: negative controls. In life-science experiments, negative controls play critical roles. In my view, they are more important than the positive controls! They form such a rich topic that we will spend at least a few episodes on discussing them. Today, we introduce basic aspects: 1) what the negative controls do in life-science experiments, 2) our practical definition by modifying the typical and ideal one, 3) how the negative control was used in our milestone graph (Fig. 3D of milestone article 1 by the Laureates of the Nobel Prize in Physiology or Medicine 2023), and 4) two critical assumptions for negative controls to work properly.
This is Part 11 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023."
(My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)
51 قسمت
اشتراک گذاریManage episode 409865497 series 3493940
محتوای ارائه شده توسط Dr. Synaptologica. تمام محتوای پادکست شامل قسمت‌ها، گرافیک‌ها و توضیحات پادکست مستقیماً توسط Dr. Synaptologica یا شریک پلتفرم پادکست آن‌ها آپلود و ارائه می‌شوند. اگر فکر می‌کنید شخصی بدون اجازه شما از اثر دارای حق نسخه‌برداری شما استفاده می‌کند، می‌توانید روندی که در اینجا شرح داده شده است را دنبال کنید.https://fa.player.fm/legal
Today's focus: negative controls. In life-science experiments, negative controls play critical roles. In my view, they are more important than the positive controls! They form such a rich topic that we will spend at least a few episodes on discussing them. Today, we introduce basic aspects: 1) what the negative controls do in life-science experiments, 2) our practical definition by modifying the typical and ideal one, 3) how the negative control was used in our milestone graph (Fig. 3D of milestone article 1 by the Laureates of the Nobel Prize in Physiology or Medicine 2023), and 4) two critical assumptions for negative controls to work properly.
This is Part 11 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023."
(My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)
51 قسمت
Alla avsnitt
×
F
Foundational Skills in Life Sciences
My students selected a paper to read in a journal club. The abstract (and the rest of the paper) was not written well. The abstract gave us two precious lessons. Lesson 1: Good structural organization will help the readers understand the content. Lesson 2: Abstracts of peer-reviewed papers can be disorganized. I hope you learn them in this episode, too! (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
We have reached 50 episodes! Thank you for listening. Today, we will briefly talk about how I have kept sustainable podcasting so far. The most important thing may be that I have set my goal simple and single: It is to create high-quality content for you. That’s it. Here are links to two sources of podcast statistics that I mentioned in the episode. https://www.amplifimedia.com/blogstein-1/lyspqop3ylro9a2t7y2de820uwkgwx https://podcastindex.org/stats (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 49. Another well-crafted logic loop in Abstract by Nobel Laureates (mini-series: reading-30) 18:26
We will talk about the second of the two, well-crafted loops of logic, in the Abstract of a paper written by the Nobel Prize Laureates. Not all abstracts have this second loop. But when it is present, it gives a better overview of the impact of the presented work. We can find one of the best examples in our Abstract. We are reading the paper written by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman, that led to the Nobel Prize in Physiology or Medicine 2023. We call it the Milestone Article 1. Here are two sets of links related to the paper. Milestone Article 1: “Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA” by Karikó, Buckstein, Ni & Weissman. Immunity, 23(2): 165-75, 2005. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ Copyright of the paper (as of March, 2025): See the pull-down menu “Article Info” near the top of the article webpage (Use the DOI above to reach it). Check the “User License” section which lists the “Elsevier user license”: --- http://www.elsevier.com/open-access/userlicense/1.0/ --- https://www.elsevier.com/about/policies-and-standards/open-access-licenses/elsevier-user For finding our Milestone Articles 1, 2 and 3 (i.e., three papers written by the Laureates that led to the Nobel Prize), visit the following website that shows the “Press Release” of the Nobel Prize in Physiology or Medicine 2023. Look for "Key publications" section. https://www.nobelprize.org/prizes/medicine/2023/press-release/ We discussed how to find Milestone Articles 1~3 as above, in Episode #10. Here is the link to the episode: https://synaptologica.buzzsprout.com/2219599/episodes/14122755 (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
I can visualize two well-crafted loops of logic, in the Abstract of a paper written by the Nobel Prize Laureates. Can you? We will talk about one of them in today’s episode. Such a loop, together with a linear progression, highlights the authors’ clear and logical thought process. We are reading the paper written by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman, that led to the Nobel Prize in Physiology or Medicine 2023. We call it the Milestone Article 1. Here are two sets of links related to the paper. Milestone Article 1: “Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA” by Karikó, Buckstein, Ni & Weissman. Immunity, 23(2): 165-75, 2005. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ Copyright of the paper (as of March, 2025): See the pull-down menu “Article Info” near the top of the article webpage (Use the DOI above to reach it). Check the “User License” section which lists the “Elsevier user license”: --- http://www.elsevier.com/open-access/userlicense/1.0/ --- https://www.elsevier.com/about/policies-and-standards/open-access-licenses/elsevier-user (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
Let's analyze a single sentence in the Abstract of an impressive paper. It is the paper that led to the 2023 Nobel Prize in Physiology or Medicine. This is the paper that we call the milestone article 1 (Please see below for details). We will analyze the second sentence. Why is this sentence important? We will discuss four of my approaches to see that this sentence is important, beautiful and helpful for readers outside the field. It is fun to spend one episode for just a single sentence, and think deep about how it reveals the authors' logic. Here are two sets of links related to the paper written by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. Milestone Article 1: “Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA” by Karikó, Buckstein, Ni & Weissman. Immunity, 23(2): 165-75, 2005. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ Copyright of the paper (as of March, 2025): See the pull-down menu “Article Info” near the top of the article webpage (Use the DOI above to reach it). Check the “User License” section which lists the “Elsevier user license”: --- http://www.elsevier.com/open-access/userlicense/1.0/ --- https://www.elsevier.com/about/policies-and-standards/open-access-licenses/elsevier-user (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 46. Let’s analyze the Abstract of a paper written by Nobel Laureates (mini-series: reading-27) 27:59
Today, we will analyze the Abstract of an impressive paper. It is the paper that led to the 2023 Nobel Prize in Physiology or Medicine. This is the paper that we call the milestone article 1. We will have two essential pre-analysis sub-steps, and then we will analyze each sentence. During this analysis, we will read each sentence of the Abstract, examine the role of each sentence, and assign each sentence to a structural component, so that we will be able to understand the authors’ intention for each sentence. Here are two sets of links mentioned in this episode. All are related to the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. Milestone Article 1: “Suppression of RNA recognition by Toll-like receptors: the impact of nucleoside modification and the evolutionary origin of RNA” by Karikó, Buckstein, Ni & Weissman. Immunity, 23(2): 165-75, 2005. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ Copyright of the paper (as of March, 2025): See the pull-down menu “Article Info” near the top of the article webpage (Use the DOI above to reach it). Check the “User License” section which lists the “Elsevier user license”: --- http://www.elsevier.com/open-access/userlicense/1.0/ --- https://www.elsevier.com/about/policies-and-standards/open-access-licenses/elsevier-user (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
Today, we will take a significant step towards understanding the 2023 Nobel Prize in Physiology or Medicine. We will read the Abstract of the Laureates’ most important paper that led to the Nobel Prize. This is the paper that we call the milestone article 1. We will have two essential pre-reading sub-steps, and then we will conduct the initial readings. These sub-steps will set the stage for a more in-depth exploration in the next episode. Here are two sets of links mentioned in this episode. All are related to the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. YouTube video of Nobel announcement (starting at ~2 minutes and 20 seconds) Embedded in Nobel website: https://www.nobelprize.org/prizes/medicine/2023/prize-announcement/ Standalone with time stamp: https://www.youtube.com/watch?v=0JP-yDsLc3k&t=2m20s Milestone Article 1: Immunity, 2005 DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
How are you reading life-science textbooks? My approach to reading textbooks has evolved significantly throughout my academic career, from student to researcher to instructor to course director. Today’s episode is part 2. We will discuss the last two phases of my experience. I am now in the fourth phase. I fully appreciate how well the authors summarized a vast amount of knowledge in one field of life sciences, in coherent and consistent manners. Please enjoy listening to my different phases of reading textbooks. It will be great if these episodes serve in stimulating your thoughts about how to read textbooks. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
How are you reading life-science textbooks? My approach to reading textbooks has evolved significantly throughout my academic career, from student to researcher to instructor to course director. Today’s episode is part 1. We will discuss the first two phases of my experience. My overall goal of the two-part episodes is to highlight the unique roles that textbooks play, different from those of primary research papers. Knowing these different purposes will help us read textbooks more effectively. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
Happy Holidays to you, and I wish you the very best in 2025!
F
Foundational Skills in Life Sciences
“Should I exclude irrelevant experiences from my academic CV?” This is a question that students often ask. In this episode, I would like to give you my thoughts about this question. In general, there is a way of creating a comprehensive CV: it will show that you have unique experiences, and you are a careful and a thoughtful writer. The key would be for you to be proud of what you did in the past. I hope that this discussion will be useful for you. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
“Should my CV be short or long, when I apply for a research assistant position in a college/university lab?” This is a question that students often ask. In this episode, I would like to give you my thoughts about this question. The content in a CV depends on the person’s career stage. But I have one common viewpoint for CVs at all stages. I would like to view the descriptions in the CV, during and after the undergraduate education, as the defining statements of the person. My thoughts about today’s question will be related to this viewpoint. At the same time, I agree that different professors and different students may have opinions different from mine. Let’s discuss them and share diverse perspectives, so that our thoughts will become deeper. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
How do top-tier scientific journals, like Nature, make sure their abstracts are clear and impactful? In today’s episode, we will identify the eight key components that make those abstracts effective, by naming the components in our own words, and clarifying their roles and order. Our system was inspired by the Nature document for the Abstract guidelines that we discussed in the Episode #38. Here is the URL that brings you directly to the Nature document: https://www.nature.com/documents/nature-summary-paragraph.pdf (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
What is the best way to learn how to read the Abstract of a scientific paper? In my view, the best way is to learn how the Abstract is structured in any paper. Today, we will learn it, by reading a very short document. It is a one-page document that describes the Abstract guidelines, provided by the journal, Nature. Here is the URL that brings you directly to the Nature document: https://www.nature.com/documents/nature-summary-paragraph.pdf Here is the URL of the webpage for the Instructions for Authors by Nature. The above document can be found as the hyperlink to the note, “annotated example” in the “Articles” section: https://www.nature.com/nature/for-authors/formatting-guide Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
The 2024 Nobel Prize was announced! Two days ago, it was in Physiology or Medicine. Yesterday, it was in Physics. Today, it was in Chemistry. The Nobel Prize in Chemistry was awarded to Dr. David Baker “for computational protein design”, and to Dr. Demis Hassabis and Dr. John Jumper, “for protein structure prediction.” Their work is directly related to life sciences. Let’s learn about the work by watching a YouTube video. It is the archived video of the announcement of The Nobel Prize. I am sure you enjoy listening to podcasts. I am also happy to report to you that Dr. David Baker launched “The Baker Lab Podcast” just two weeks before this recording! Here is the URL of the YouTube video: https://www.youtube.com/watch?v=bPjB9NRu8Jc Here is the URL of the same video, but with a timestamp at the start of slide presentation: https://www.youtube.com/watch?v=bPjB9NRu8Jc&t=4m40s Here is the URL of the same video, but embedded on the Nobel Prize website: https://www.nobelprize.org/prizes/chemistry/2024/prize-announcement/ Here is the URL of a new podcast, “The Baker Lab Podcast” hosted by Dr. David Baker: https://www.bakerlab.org/podcast/ Enjoy the video and the podcast! (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
The 2024 Nobel Prize was announced! Yesterday, it was in Physiology or Medicine. Today it was in Physics. It was awarded to Dr. John Hopfield, and Dr. Geoffrey Hinton, “for foundational discoveries and inventions that enable machine learning with artificial neural networks.” It is somewhat (indirectly) related to my field, neuroscience! Let’s learn about the work by watching a YouTube video. It is the archived video of the announcement of The Nobel Prize. Here is the URL of the YouTube video: https://www.youtube.com/watch?v=SBGG4WNweEc Here is the URL of the same video, but embedded on the Nobel Prize website: https://www.nobelprize.org/prizes/physics/2024/prize-announcement/ Enjoy the video! (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
The 2024 Nobel Prize was announced! This year’s Nobel Prize in Physiology or Medicine was awarded to Dr. Victor Ambros, and Dr. Gary Ruvkun, “for the discovery of microRNA and its role in post-transcriptional gene regulation.” Let’s learn about the work by watching a YouTube video. It is the archived video of the announcement of The Nobel Prize. Here is the URL of the YouTube video: https://www.youtube.com/watch?v=Ln5rCmDqua0 Here is the URL of the same video, but embedded on the Nobel Prize website: https://www.nobelprize.org/prizes/medicine/2024/prize-announcement/ Enjoy the video! (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 34. Lessons learned from Nobel Laureates' work, about positive and negative controls (mini-series: reading-23) 31:10
We will complete the analysis of positive and negative controls in the milestone article of the Nobel Laureates of 2023. The article shows us one of the best examples of how to use positive and negative controls in life-science experiments. This episode will be Part 2 of a 2-part summary of control experiments. This will also conclude Step 11 of reading the article, which is to examine the key graph of the article. We can learn a lot from the Nobel Laureates! Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ Here are the links to the YouTube video of Nobel announcement. Embedded on Nobel website: https://www.nobelprize.org/prizes/medicine/2023/prize-announcement/ Standalone with time stamp: https://www.youtube.com/watch?v=0JP-yDsLc3k&t=2m20s (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
Question of today: What is a practical tip for effective oral presentations? There are many important things to do, for delivering oral presentations successfully. But I will be happy to give you my number one tip and the reasons why. And this tip is not discussed often in scientific communities. I hope you will find it useful! Disclaimer: What I'm going to tell you is my personal opinion. It is possible that it does not apply to you in your own specific situation, in your program, in your own institution, and in your country. And each professor could have a different opinion. So, please listen to my comment, not as definitive advice, but just as a reference. I hope my comment will still serve you as a starting point for your thought. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 32. Is it important to include positive and negative controls in each experiment? Lesson from Nobel Laureates' work (mini-series: reading-22) 21:32
We will examine the positive and negative controls in the milestone article of the Nobel Laureates. The article shows us one of the best examples of how to use positive and negative controls in life-science experiments. We will compare a total of eight figure panels. And we will learn important lessons from them, for designing experiments and for reading figures. This episode will be Part 1 of a summary of control experiments. Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
The first Q&A session! Question of today: Is it OK to say, “I don’t know” in response to technical questions in an interview when you apply for a life-science research position in a laboratory? I will give you my answer and the reasons why. Disclaimer: What I'm going to tell you is my personal opinion. It is possible that it does not apply to you in your own specific situation, in your program, in your own institution, and in your country. And each professor could have a different opinion. So, please listen to my comment, not as definitive advice, but just as a reference. I hope my comment will still serve you as a starting point for your thought. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
We will keep analyzing the specific positive controls that the Nobel Laureates used in their milestone article 1 that led to the Nobel Prize. We will examine the four positive controls in the third figure of this paper, and in other figures as well. Those positive controls did not necessarily show positive responses in multiple figures. We will observe that positive controls can show variable behaviors in a paper. We will also discuss how a modified definition of positive controls will be helpful for understanding them. Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
Let’s analyze the specific positive controls that the Nobel Laureates used in their milestone article 1 that led to the Nobel Prize. We will look at the first two figures and four positive controls therein. As always, we are impressed by the meticulous design of the experiments by the Laureates. Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
In this episode, we shift our focus from negative controls to their counterparts: positive controls. We will begin by defining what we mean by positive controls. Then we will refine that definition to encourage practical and cautious thinking. Here are the links to the Milestone Article 1 (Immunity, 2005) by the Laureates, Dr. Katalin Karikó & Dr. Drew Weissman of the Nobel Prize in Physiology or Medicine 2023. DOI: https://doi.org/10.1016/j.immuni.2005.06.008 PDF: https://www.cell.com/immunity/pdf/S1074-7613(05)00211-6.pdf PubMed: https://pubmed.ncbi.nlm.nih.gov/16111635/ (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
One-year anniversary of this podcast channel! Thank you for listening. Why did I start podcasting? The motivation or the trigger was the students’ feedback to my teaching and training: they loved it. My goal is to help you learn and polish your skills in life sciences. In this episode, I will talk about a little story of how I started podcasting. (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
This episode continues from the last one. This will mark the end of our story about the life-science experiment that did not work at first, but worked well after extensive troubleshooting. We will talk about the remaining three lessons we can learn from the story. They will be about thoughtful approaches, and about probably the most important mindset in life sciences (What will it be?). This is Part 18 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023." (My email is active: ideas@synaptologica.com. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
In the last episode, we finished discussing details of the life-science experiments in our story. We observed that negative controls can be powerful tools for troubleshooting and ensuring data quality. The story gave us invaluable learning experience. What lessons can we learn? We will talk about three lessons related to negative controls today. They will help us design good research and evaluate results well. This is Part 17 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023." (My email is active: ideas@synaptologica.com. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 24. Negative controls put the pieces together in troubleshooting experiments (mini-series: reading-16) 46:41
We will see how the experimenters implemented a good, troubleshooting idea into a protocol that appeared to be challenging at first. We are continuing to discuss the troubleshooting of a real, life-science experiment in our story. Sophisticated thought processes made the experiment possible in the end. This episode celebrates the power of well-designed experiments. We'll see how meticulous planning and a systematic approach to troubleshooting can overcome seemingly insurmountable obstacles. This is Part 16 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023." (My email is active: ideas@synaptologica.com. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 23. Refining a troubleshooting idea: the power of identifying unknowns (mini-series: reading-15) 25:11
We will discuss the fourth and final idea, for troubleshooting a real, life-science experiment in our story. This idea was based on the previous three ideas. Then the experimenters took an impressive step. They identified key unknowns about the experiment, and refined and updated their idea. The updated idea laid the foundation for designing the crucial, fourth and final troubleshooting experiment that we will discuss in the next episode. This is Part 15 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023." (My email is active: ideas@synaptologica.com. But my website is under construction. Please wait for a while. Thank you for the patience!)…
F
Foundational Skills in Life Sciences
1 22. Negative controls unveil a hidden culprit in a problematic experiment (mini-series: reading-14) 14:39
We continue our deep exploration into negative controls and their importance in troubleshooting life-science experiments. We follow a real-life story of an experiment that yielded unexpected results. Through the third troubleshooting experiment utilizing negative controls, the experimenters finally uncovered the culprit. We discuss how negative controls helped identify the problem and pave the way for further investigation. This is Part 14 of the reading mini-series "Let’s read a paper written by Nobel Prize Laureates, 2023." (My email is active. But my website is under construction. Please wait for a while. Thank you for the patience!)…
به Player FM خوش آمدید!
Player FM در سراسر وب را برای یافتن پادکست های با کیفیت اسکن می کند تا همین الان لذت ببرید. این بهترین برنامه ی پادکست است که در اندروید، آیفون و وب کار می کند. ثبت نام کنید تا اشتراک های شما در بین دستگاه های مختلف همگام سازی شود.
Player FM - برنامه پادکست
با برنامه Player FM !
با برنامه Player FM !
))
