For centuries, members of the B’doul Bedouin tribe lived in the caves around the ancient city of Petra, Jordan. Then, in the 1980s, the government forced the tribe to move in the name of preserving the geological site for tourists. But if the residents are forced to leave, and if their heritage has been permanently changed, then what exactly is being preserved? SHOW NOTES: Meet The Man Living in The Lost City Carved in Stone Jordan: Petra's tourism authority cracks down on Bedouin cave dwellers The tribes paying the brutal price of conservation “There is no future for Umm Sayhoun” Jordan’s Young Bedouins Are Documenting Their Traditions on TikTok Check out Sami's company Jordan Inspiration Tours Learn about your ad choices: dovetail.prx.org/ad-choices…
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Optimizing Nuclei Extraction & Counting for Single Cell Sequencing
Manage episode 433812483 series 3391756
محتوای ارائه شده توسط Bitesize Bio. تمام محتوای پادکست شامل قسمت‌ها، گرافیک‌ها و توضیحات پادکست مستقیماً توسط Bitesize Bio یا شریک پلتفرم پادکست آن‌ها آپلود و ارائه می‌شوند. اگر فکر می‌کنید شخصی بدون اجازه شما از اثر دارای حق نسخه‌برداری شما استفاده می‌کند، می‌توانید روندی که در اینجا شرح داده شده است را دنبال کنید.https://fa.player.fm/legal
Single cell sequencing samples must be prepared to the highest possible standard.
Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing.
In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample.
You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.
Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
138 قسمت
اشتراک گذاریManage episode 433812483 series 3391756
محتوای ارائه شده توسط Bitesize Bio. تمام محتوای پادکست شامل قسمت‌ها، گرافیک‌ها و توضیحات پادکست مستقیماً توسط Bitesize Bio یا شریک پلتفرم پادکست آن‌ها آپلود و ارائه می‌شوند. اگر فکر می‌کنید شخصی بدون اجازه شما از اثر دارای حق نسخه‌برداری شما استفاده می‌کند، می‌توانید روندی که در اینجا شرح داده شده است را دنبال کنید.https://fa.player.fm/legal
Single cell sequencing samples must be prepared to the highest possible standard.
Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing.
In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample.
You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist.
Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join
Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/
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1 AI-Powered 3D Organoid Analysis 1:02:09
1:02:09 
پخش در آینده 
پخش در آینده 
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دوست داشته شد1:02:09
پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شدMulticolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology. This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids. See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals. And get a showcase of how AIVIA supports Python-based image analysis for microscopists. Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysis Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings. Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL. Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Learn more about the capabilities of MULTI-seq in single-cell sequencing in this episode of Listen In . Developed by the Gartner Lab at UCSF , this innovative multiplexing method increases sample and cell throughput while minimizing cost and improving your data quality. From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research. Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In , hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development. We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/ "Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
1 Enhancing 3D Spatial Biology with AI: Simplified Insights for All 1:09:11
1:09:11 پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شد1:09:11
پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شدIn this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers. See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia. Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations. You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context. We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms. Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
1 Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies 1:12:31
1:12:31 پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شد1:12:31
پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شدImaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research. In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation. Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions. Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results. Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state. In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements. In this episode, hear from immunoassay experts as they guide you through all these areas. Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
1 Windows On Neurovascular Pathologies 1:03:18
1:03:18 پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شد1:03:18
پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شدThe brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing. In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling. Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states. In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions. Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3. Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In , discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously. Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies. Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis. However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material. In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies. You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development. Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
1 Multiplex Annotated Tissue Imaging System (MANTIS®) 1:07:54
1:07:54 پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شد1:07:54
پخش در آینده پخش در آینده لیست ها پسندیدن دوست داشته شدJoin us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system. Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice. Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy. Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen. In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper. These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers. Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art. Tune in to discover how you can turn your passion for science and art into a fulfilling career. Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching. Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels. Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training. Watch the full presentation here: https://microscopyfocus.com/five-color-sted/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…
Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice. Learn how to tackle issues like: • Career disruption • Reason for choosing your place of work • Track record relative to opportunity • Consumer involvement This episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant! Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com…
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